Anti-c-myc epitope tag [9E10]

SKU: orb396136

Description

Mouse monoclonal antibody to c-myc epitope tag.

Research Area

Cancer, Epigenetics

Images & Validation

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Item 1 of 2

Tested Applications	IF, IHC-Fr, IHC-P, IP, WB
Reactivity	Human

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	F(ab)2
Clone No.	9E10
Immunogen	A synthetic peptide corresponding to aa 408-439 from C-terminus of human c-myc.
Target	c-myc epitope tag
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Proto-oncogene c-Myc; Transcription factor p64; Class E basic helix-loop-helix protein 39; BHLHE39; cmyc

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ELISA of anti-epitope tag antibodies on multi-tag protein Plate coated with 5 ug/ml of Multitag protein. Primary antibody loaded at 1000 ng/ml in first well and an 11 point serial dilution performed in duplicate for each antibody with last well left blank. Signal detection was performed using HRP-conjugated goat anti-mouse.

Left Panel: orb256348 (2 µg/ml) staining of HEK293 spiked (A) and unspiked (B) with 5 ng multitag protein (35 µg protein in RIPA buffer). Lane C contains 25 ng purified multitag protein as positive control. Right Panel: 1 µg/ml staining of Multitag protein at 25 ng (A), 10 ng (B) and 5 ng (C) amounts. For both, primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

c-myc epitope tag

UniProt

P01106

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

Immunofluorescence

Immunoprecipitation