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Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 3
| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution Range | WB: 1:1000-1:3000, IHC-P: 1:100-1:200, IP: 1:50-1:200 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence of human Carbonic Anhydrase 9. The exact sequence is proprietary. |
| Target | CA9 |
| Purification | Affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−G250; MN; Carbonic anhydrase 9; Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1
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Western blot analysis of Carbonic Anhydrase 9 expression in Hela (A), 293T (B) whole cell lysates. (Predicted band size: 49 kD; Observed band size: 49 kD)
Immunohistochemical analysis of Carbonic Anhydrase 9 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunoprecipitation of Carbonic Anhydrase 9 from 0.5 mg Hela whole cell extract lysate, using Anti-Carbonic Anhydrase 9 Antibody Antibody.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
Carbonic Anhydrase 9 Mouse Monoclonal Antibody (orb323113)
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