Caspase 1 p10 Rabbit Polyclonal Antibody

SKU: orb213638

Description

The Caspase 1 p10 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Caspase 1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

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Item 1 of 3

Tested Applications	IF, WB
Dilution Range	WB: 1-500-1-1000, IF/ICC: 1-100-1-500
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Caspase 1. The exact sequence is proprietary.
Target	CASP1
Purification	The antibody was purified by immunogen affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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IL1BC; IL1BCE; Caspase-1; CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45

Quality Guarantee

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Caspase 1 p10 Rabbit Polyclonal Antibody

Western blot analysis of Caspase 1 p10 expression in human spleen (A) whole cell lysates. (Predicted band size: 45 kD; Observed band size: 45 kD)

Western blot analysis of Caspase 1 p10 expression in H1792 (A), MCF7 (B), Hela (C), mouse spleen (D), mouse lung (E), rat spleen (F) whole cell lysates.

Immunofluorescent analysis of Caspase 1 p10 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

CASP1

UniProt

P29466, P29452, P43527

NCBI Gene ID

834, 12362

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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