Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	A synthetic peptide corresponding to a sequence at C-terminus of human CCR5, which shares 76.2% and 85.7% amino acid (aa) sequence identity with mouse and rat CCR5, respectively.
Target	C-C chemokine receptor type 5
Molecular Weight	41 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Concentration	500 µg/ml
Disclaimer	For research use only

Alternative Names

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C C chemokine receptor type 5; C C CKR 5; CC CKR 5; CCCKR5; CCR 5; CCR5; CD195; CHEMR13; CKR 5; CKR5; CMKBR5; HIV 1 fusion coreceptor; IDDM22

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Western blot analysis of CCR5 using anti-CCR5 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR5 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CCR5 at approximately 41 kDa. The expected band size for CCR5 is at 41 kDa.

Quick Database Links

Gene Symbol

C-C chemokine receptor type 5

UniProt

P51681

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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