CD122 Rat Monoclonal Antibody

SKU: orb1293377

Description

The CD122 Antibody is suitable for FC, IF. It is a Monoclonal, Unconjugated antibody which raised against Rat T cell line expressing mouse CD122 ( IL-2Rβ) .Purification: The antibody was purified by affinity chromatography.

Images & Validation

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Tested Applications	FC, IF, WB
Dilution Range	WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)
Reactivity	Mouse
Application Notes	1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-rat IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured). Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Rat
Clonality	Monoclonal
Isotype	Rat IgG2b kappa
Clone No.	TM-β1
Immunogen	Rat T cell line expressing mouse CD122 ( IL-2Rβ)
Target	IL2RB
Purification	The antibody was purified by affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	PBS, pH 7.3, and 0.02% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Interleukin-2 receptor subunit beta; IL-2 receptor subunit beta; IL-2R subunit beta; IL-2RB; High affinity IL-2 receptor subunit beta; p70-75; p75; CD122

Quality Guarantee

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Quick Database Links

Gene Symbol

IL2RB

UniProt

P16297

NCBI Gene ID

16185

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

Documents Download

Datasheet

Product Information

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Protocol Information

Western Blot (IB, immunoblot)

Flow Cytometry

Immunofluorescence