CD13 Mouse Monoclonal Antibody (Biotin)

SKU: orb1294195

Description

The CD13 Antibody-Biotin labled is suitable for FC, IF. It is a Monoclonal, Biotin conjugated antibody which raised against Native purified human CD13.. Purification: The antibody was purified by affinity chromatography.

Research Area

Cardiovascular Research; Immunology & Inflammation

Images & Validation

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Tested Applications	FC, IF
Dilution Range	10 μl / assay
Reactivity	Human
Application Notes	1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature. 7. Repeat step 5. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured). Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2b
Clone No.	46A11E3
Immunogen	Native purified human CD13.
Target	ANPEP
Purification	The antibody was purified by affinity chromatography.
Conjugation	Biotin

Storage & Handling

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Storage	Shipped and store at 4°C for one year. Do not freeze.
Form/Appearance	Liquid
Buffer/Preservatives	PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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APN; CD13; PEPN; Aminopeptidase N; AP-N; hAPN; Alanyl aminopeptidase; Aminopeptidase M; AP-M; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; gp150; CD antigen CD13

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Quick Database Links

Gene Symbol

ANPEP

UniProt

P15144

NCBI Gene ID

290

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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Immunofluorescence

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