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Anti-CD131 [BION-1]
SKU: orb669741
Description
Images & Validation
−Item 1 of 2
| Tested Applications | Blocking, Functional Studies, IHC, IP, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG |
| Clone No. | BION-1 |
| Immunogen | BALB/c mice were immunized intraperitonally with COS cells transfected DQP (Domain 4 of the common beta-chain) expression constructs. The immunizations were repeated 4 times at 2-week intervals. Four weeks after the final immunization, a mouse was boosted with COS transfectants intravenously. Three days later, splenocytes were harvested and fused with NS-1 myeloma cells. Hybridoma supernatants were screened on CHO DQP cells by flow cytometry, with untransfected CHO cells as a control. All antibodies were from single hybridoma clones as selected by a limiting dilution method. MoAbs were purified from ascites fluid or hybridoma supernatant by a protein A sepharose column. |
| Target | CD131 |
| Purity | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−CSF2RB; CD131; IL3RB; IL-3R common beta subunit; IL5RB; colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage); SMDP5; Common Beta-Chain; CDw131; cytokine receptor common subunit beta; beta-c; CD131; Beta Common Chain
Similar Products
−- Item 1 of 2
Anti-CD131 [BION-1] [orb669740]
Blocking, Functional Studies, IHC, IP, WB
Human
Mouse
Monoclonal
Unconjugated
0.2 mg
![Anti-CD131 [BION-1]](/images/pub/media/catalog/product/NewWebsite/35/orb669740_1.png)
![Anti-CD131 [BION-1]](/images/pub/media/catalog/product/NewWebsite/35/orb669740_2.png)
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![Anti-CD131 [BION-1]](/images/pub/media/catalog/product/NewWebsite/35/orb669741_1.png)
Flow-cytometry using the Anti-CD131 antibody BION-1. U937 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of BION-1 ((orb669741, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
![Anti-CD131 [BION-1]](/images/pub/media/catalog/product/NewWebsite/35/orb669741_2.png)
Immunofluorescence staining of U937 cells using anti-CD131 antibody BION-1. Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of BION-1 (orb669741) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb669741, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (orb256390) followed by Alexa Fluor® 488 secondary antibody.
Quick Database Links
Gene Symbol
CD131
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
Anti-CD131 [BION-1] (orb669741)
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