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CD142 Biosimilar Antibody
SKU: orb1291207
Featured
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, FC |
|---|---|
| Dilution range | ELISA 1:5000-10000, Flow Cyt 1:100 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Biosimilar Antibody |
|---|---|
| Host | Human |
| Clonality | Monoclonal |
| Isotype | Human IgG1 - kappa |
| Target | CD142 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Powder |
| Disclaimer | For research use only |
Alternative Names
−Tissue factor, TF, F3, Thromboplastin, Coagulation factor III
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ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human CD142 Protein, hFc Tag (orb1290926) can bind Anti-CD142 Neutralizing antibody (orb1291207) in a linear range of 2.93–187.50 ng/ml. In order to specifically detect orb1291207, mouse anti-human Fab-specific antibody was used as detection antibody.
Flow cytometry analysis of antigen binding of anti-human CD142 mAb (orb1291207). (A) orb1291207 does not bind to CHO-S cells that do not express CD142. (B) A clear peak shift of orb1291207 was seen compared to the control when incubated with CD142-expressing Hela cells, indicating strong binding of orb1291207 to CD142. Antibodies were incubated at 5 μg/mL.
Quick Database Links
Gene Symbol
CD142
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CD142 Biosimilar Antibody (orb1291207)
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