CD163 Mouse Monoclonal Antibody (APC)

SKU: orb1294111

Description

The CD163 Antibody-APC labled is suitable for FC, IF. It is a Monoclonal, APC conjugated antibody which raised against Human monocytes. Purification: The antibody was purified by affinity chromatography.

Research Area

Epigenetics, Immunology

Images & Validation

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Tested Applications	FC, IF
Dilution Range	10 μl / assay
Reactivity	Human
Application Notes	1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured). Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1 kappa
Clone No.	GHI/61
Immunogen	Human monocytes
Target	CD163
Purification	The antibody was purified by affinity chromatography.
Conjugation	APC

Storage & Handling

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Storage	Shipped and store at 4°C for one year. Do not freeze.
Form/Appearance	Liquid
Buffer/Preservatives	PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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M130; Scavenger receptor cysteine-rich type 1 protein M130; Hemoglobin scavenger receptor; CD163

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Quick Database Links

Gene Symbol

CD163

UniProt

Q86VB7

NCBI Gene ID

9332

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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Immunofluorescence

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