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Anti-CD19 [FMC63]
SKU: orb348921
Featured
Description
Images & Validation
−Item 1 of 2
| Tested Applications | Depletion, FACS, FC, IF, IHC, IP, In vivo |
|---|---|
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG |
| Clone No. | FMC63 |
| Immunogen | Human prolymphocytic leukaemia cell line JVM3. |
| Target | CD19 |
| Purity | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; Leu12; T-cell surface antigen Leu-12
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Anti-CD19 [FMC63] [orb348877]
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Human
Mouse
Monoclonal
Unconjugated
0.2 mg - Item 1 of 3
![Anti-CD19 [FMC63]](/images/pub/media/catalog/product/NewWebsite/35/orb348877_1.png)
![Anti-CD19 [FMC63]](/images/pub/media/catalog/product/NewWebsite/35/orb348877_2.png)
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![Anti-CD19 [FMC63]](/images/pub/media/catalog/product/NewWebsite/35/orb348921_1.png)
Flow-cytometry using anti-CD19 antibody FMC63 Human lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of FMC63 (orb348921, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
![Anti-CD19 [FMC63]](/images/pub/media/catalog/product/NewWebsite/35/orb348921_2.png)
Immunohistochemical staining of rat spleen tissue using anti-CD19 antibody FMC63. Anti-CD19 staining of formaldehyde fixed paraffin embedded rat spleen tissue, at 40x magnification. The mouse IgG 2A-chimeric version of FMC63 (orb348877) was used to stain samples at a concentration of 3 µg/ml.
Quick Database Links
Gene Symbol
CD19
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
IHC
Immunohistochemistry
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
FC
Flow Cytometry
IF
Immunofluorescence
IP
Immunoprecipitation
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Nicholson, I. C. et al. Construction and characterisation of a functional CD19 specific single chain Fv fragment for immunotherapy of B lineage leukaemia and lymphoma Mol Immunol, 34, 1157-1165 (1997)
Reactivity
Human
Anti-CD19 [FMC63] (orb348921)
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