Anti-CD205 [NLDC145]

SKU: orb1152484

Description

Rat monoclonal antibody to CD205

Images & Validation

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Item 1 of 2

Tested Applications	FC, IB, IF, IHC
Reactivity	Mouse

Key Properties

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Host	Rat
Clonality	Monoclonal
Isotype	IgG2a
Clone No.	NLDC145
Immunogen	This antibody was raised by immunising Wistar rats with mouse lymph node stroma.
Target	CD205
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Ly-75; NLDC-145; DEC205; DEC-205; Lymphocyte antigen 75; Ly-75

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Flow cytometry using the Anti-CD205 antibody NLDC145. Paraformaldehyde fixed mouse thymocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of NLDC145 (orb1152485, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of mouse thymocytes with anti-CD205 NLDC145. Immunofluorescence analysis of paraformaldehyde fixed mouse thymocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of NLDC145 (orb1152485) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1152485, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

CD205

UniProt

Q60767

UniProt Details

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No UniProt data available

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Protocol Information

IHC

Immunohistochemistry

Flow Cytometry

Immunofluorescence