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CD24 Antibody
SKU: orb1291204
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, FC, IHC, WB |
|---|---|
| Dilution range | ELISA 1/5000-10000|Flow Cyt 1/100 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit/Human Fc chimeric IgG1 |
| Clone No. | DMC213 |
| Target | CD24 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Disclaimer | For research use only |
Alternative Names
−CD24A
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Flow cytometry analysis with Anti-CD24 on Expi293 cells transfected with human CD24 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Anti-CD24 antibody (SKU# orb1291204) at 1/1000 dilution.Lane: Huh7, whole cell lysate.Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution.Predicted band size: 61 kDa.Observed band size: 25, 135 kDa
A. orb1291204 at 10 μg/ml staining CD24 in K562-CD24 cells by IHC (SKU# orb1291204); B. orb1291204 at 10 μg/ml staining CD24 in human tonsil tissue by IHC (SKU# orb1291204)
Flow cytometry analysis of antigen binding of anti-human CD24 mAb (orb1291204). (A) orb1291204 does not bind to Jurkat cells that do not express CD24. (B) A clear peak shift of orb1291204 was seen compared to the control when incubated with CD24-expressing MCF-7 cells, indicating strong binding of orb1291204 to CD24. Antibodies were incubated at 10 μg/mL.
Quick Database Links
Gene Symbol
CD24
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CD24 Antibody (orb1291204)
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