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CD30 Antibody
SKU: orb1174063
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, FC, WB |
|---|---|
| Dilution Range | ELISA 1/5000-10000|Flow Cyt 1/100 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | DM105 |
| Target | CD30 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−TNFRSF8;CD30;D1S166E;Ki-1
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ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD30 protein, His tagged protein orb689465 can bind Rabbit anti-CD30 monoclonal antibody (clone: DM105) in a linear range of 0.12-80 ng/ml.
Flow cytometry analysis with Anti-CD30 on Expi293 cells transfected with human CD30 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Flow cytometry analysis of antigen binding of rabbit anti-human CD30 mAb (orb1174063). (A) orb1174063 does not bind to 293T cells that do not express CD30. (B) A clear peak shift of orb1174063 was seen compared to the control when incubated with CD30-expressing 8226 cells, indicating strong binding of orb1174063 to CD30. Antibodies were incubated at 10 μg/mL.
Anti-CD30 antibody (SKU# orb1174063) at 1/1000 dilution.Lane: RPMI8226, whole cell lysate.Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution.Predicted band size: 64 kDa.Observed band size: 90, 110 kDa
Quick Database Links
Gene Symbol
CD30
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CD30 Antibody (orb1174063)
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