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CD33 Antibody
SKU: orb757402
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, FC |
|---|---|
| Dilution range | ELISA 1/5000-10000|Flow Cyt 1/100 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | DM77 |
| Target | CD33 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Disclaimer | For research use only |
Alternative Names
−CD33,SIGLEC3,gp67
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ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD33 protein, hFc-His tagged protein orb689399 can bind Rabbit anti-CD33 monoclonal antibody (clone: DM77) in a linear range of 1-100 ng/ml.
Expi 293 cell line transfected with irrelevant protein (A) and human CD33 (B) were surface stained with Rabbit anti-CD33 monoclonal antibody 1 μg/ml (clone: DM77) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Flow cytometry data of serially titrated Rabbit anti-CD33 monoclonal antibody (clone: DM77) on Expi 293 cell line transfected with human CD33. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Flow cytometry analysis of antigen binding of rabbit anti-human CD33 mAb (orb757402). (A) orb757402 does not bind to 293T cells that do not express CD33. (B) A clear peak shift of orb757402 was seen compared to the control when incubated with CD33-expressing THP-1 cells, indicating strong binding of orb757402 to CD33. Antibodies were incubated at 10 μg/mL.
Quick Database Links
Gene Symbol
CD33
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CD33 Antibody (orb757402)
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