CD45 Mouse Monoclonal Antibody (APC/Cy7)

SKU: orb1294038

Description

The CD45 Antibody-APC/Cy7 labled is suitable for FC, IF. It is a Monoclonal, APC/Cy7 conjugated antibody which raised against Native purified human CD45.. Purification: The antibody was purified by affinity chromatography.

Research Area

Epigenetics, Infectious Diseases

Images & Validation

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Tested Applications	FC, IF
Dilution Range	10 μl / assay
Reactivity	Human
Application Notes	1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured). Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1 kappa
Clone No.	HI30
Immunogen	Native purified human CD45.
Target	PTPRC
Purification	The antibody was purified by affinity chromatography.
Conjugation	APC/Cy7

Storage & Handling

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Storage	Shipped and store at 4°C for one year. Do not freeze.
Form/Appearance	Liquid
Buffer/Preservatives	PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD45; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; CD antigen CD45

Quality Guarantee

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Quick Database Links

Gene Symbol

PTPRC

UniProt

P08575

NCBI Gene ID

5788

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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Immunofluorescence

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