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CD45 Antibody
SKU: orb1474239
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | WB (1/500 - 1/1000), IH (1/50 - 1/100) |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | Purified recombinant fragment of human PTPRC (AA: 928-989) expressed in E. Coli. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD45; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; CD45
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Western blot analysis of CD45 expression in Ramos (A), Raw264.7 (B) whole cell lysates. (Predicted band size: 147 kD; Observed band size: 180-240kD)
Immunohistochemical analysis of CD45 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.34). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
CD45 Antibody (orb1474239)
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