Cd59 Rabbit Polyclonal Antibody

SKU: orb669165

Description

Anti-Cd59 Antibody. Tested in IHC applications. This antibody reacts with Mouse, Rat.

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Metabolism Research, Microbiology, Protein Biochemistry, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 2

Tested Applications	IHC
Dilution range	Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	A synthetic peptide corresponding to a sequence in the middle region of rat Cd59, which shares 66.7% amino acid (aa) sequence identity with mouse Cd59.
Target	CD59 glycoprotein
Molecular Weight	20 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Concentration	500 µg/ml
Disclaimer	For research use only

Alternative Names

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16.3A5; 1F5; 1F5 antigen; CD59; CD59 glycoprotein; EJ16; EJ30; EL32; G344; HRF 20; HRF20; MAC inhibitory protein; MAC IP; MACIF; MEM43; MEM43 antigen; MIC11; MIN1; MIN2; MIN3; MIRL; MSK21; p18 20; Protectin

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IHC analysis of Cd59 using anti-Cd59 antibody. Cd59 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Cd59 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of Cd59 using anti-Cd59 antibody. Cd59 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Cd59 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Quick Database Links

Gene Symbol

CD59 glycoprotein

UniProt

P27274

UniProt Details

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No UniProt data available

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Protocol Information

IHC

Immunohistochemistry

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