Anti-CD79b [HM79-16]

SKU: orb758942

Description

Hamster monoclonal antibody to CD79b

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 3

Tested Applications	FC, IF, IHC, IP
Reactivity	Mouse

Key Properties

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Host	Hamster
Clonality	Monoclonal
Isotype	IgG
Clone No.	HM79-16
Immunogen	HM79-16 was initially generated by purifying CD79b from WEHI231 B cells and used for immunisation of footpads of an Armenian hamster. Hybridoma supernatants were screened for positive reactivity in surface immunofluorescence of WEHI231 and then the specificity to CD79b was tested by immuno-precipitationRecombinant anti-CD79 antibodies were engineered by cloning and sequencing the full-length IG heavy and light chains of an Armenian hamster (Cricetulus migratorius).
Target	CD79b
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Ig-beta; AGM6; B29; IGB; CD79b molecule;

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Flow cytometry using the Anti-CD79b antibody HM79-16. Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of HM79-16 (orb758943, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed mouse splenocytes with anti-CD79b antibody HM79-16. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version HM79-16 (orb758943) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758943, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CD79b antibody HM79-16 Mouse spleen tissue lysates (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HM79-16 (orb758943) at 0.1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Quick Database Links

Gene Symbol

CD79b

UniProt

P15530

UniProt Details

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No UniProt data available

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Protocol Information

IHC

Immunohistochemistry

Flow Cytometry

Immunofluorescence

Immunoprecipitation