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CD8 Cd8A Mouse Monoclonal Antibody
Description
Research Area
Images & Validation
−| Tested Applications | FC, ICC, IHC |
|---|---|
| Dilution Range | Immunohistochemistry (Frozen Section), 1μg/ml, Human Immunocytochemistry, 0.5-1μg/ml, Human Flow Cytometry, 1-3μg/1x10^6 cells, Human |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a |
| Clone No. | CA-8 |
| Immunogen | Human thymocytes followed by peripheral blood T cells. |
| Target | T-cell surface glycoprotein CD8 alpha chain |
| Molecular Weight | 25 kDa |
| Purification | Ascites |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required. |
| Concentration | Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Flow Cytometry analysis of human PBMC cells using anti-CD8 antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

IHC analysis of CD8 using anti-CD8 antibody.CD8 was detected in paraffin-embedded section of human tonsil tissues.
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Protocol Information
CD8 Cd8A Mouse Monoclonal Antibody (orb18195)
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