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CD8a Antibody
SKU: orb179912
Description
Research Area
Epigenetics, Immunology
Images & Validation
−Item 1 of 2
| Tested Applications | FC, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Reactivity | Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 kappa |
| Clone No. | OX-8 |
| Immunogen | High Mw glycoproteins from rat thymocytes |
| Target | CD8a |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Ly-2, Ly-B, Ly-35, Lyt-2
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Separation of rat CD8a positive thymocytes (red-filled) from CD8a negative thymocytes (black-dashed) in flow cytometry analysis (surface staining) of rat thymocyte suspension stained using anti-rat CD8a (OX-8) purified antibody (concentration in sample 0.32 μg/ml) GAM APC.
Flow cytometry surface staining pattern of rat thymocyte suspension stained using anti-rat CD8a (OX-8) purified antibody (concentration in sample 0.32 μg/ml) GAM APC.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IP
Immunoprecipitation
CD8a Antibody (orb179912)
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