CD86 Rabbit Polyclonal Antibody

SKU: orb377982

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Description

The CD86 Antibody is suitable for FC, IF, IHC, IP, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human CD86 .Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics, Immunology, Infectious Diseases

Images & Validation

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Item 1 of 2

Tested Applications	FC, IHC, IP, WB
Dilution Range	WB (1/500 - 1/1000), IH (1/50 - 1/200), IP (1/10 - 1/50), FC (1/10 - 1/50)
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant fusion protein of human CD86
Target	CD86
Purification	The antibody was purified by immunogen affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD28LG2; T-lymphocyte activation antigen CD86; Activation B7-2 antigen; B70; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD86

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Western blot analysis of CD86 expression in mouse thymus (A), mouse spleen (B), rat spleen (C), rat lung (D) whole cell lysates. (Predicted band size: 12; 24; 28; 31; 37 kD; Observed band size: 72 kD)

Immunofluorescent analysis of CD86 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

CD86

UniProt

P42081, P42082

NCBI Gene ID

942, 12524

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

Flow Cytometry

Immunoprecipitation