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CD86 Antibody
SKU: orb377982
Featured
Description
Images & Validation
−Item 1 of 2
| Tested Applications | FC, IF, IHC, IP, WB |
|---|---|
| Dilution range | WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200), IP (1/10 - 1/50), FC (1/10 - 1/50) |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Recombinant fusion protein of human CD86 |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Disclaimer | For research use only |
Alternative Names
−CD28LG2; T-lymphocyte activation antigen CD86; Activation B7-2 antigen; B70; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD86
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Western blot analysis of CD86 expression in mouse thymus (A), mouse spleen (B), rat spleen (C), rat lung (D) whole cell lysates. (Predicted band size: 12; 24; 28; 31; 37 kD; Observed band size: 72 kD)
Immunofluorescent analysis of CD86 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
IP
Immunoprecipitation
CD86 Antibody (orb377982)
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