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Anti-CD86 [PO3]
SKU: orb758797
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 1
| Tested Applications | Blocking, FA, FC, IHC, WB |
|---|---|
| Reactivity | Mouse |
Key Properties
−| Host | Rat |
|---|---|
| Clonality | Monoclonal |
| Isotype | Rat IgG |
| Clone No. | PO3 |
| Immunogen | This antibody was raised by immunising Sprague Dawley rats with a mouse B cell line, BCL1 and fusing immune splenocytes with P3U1 myeloma cells. |
| Target | CD86 |
| Purity | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−PO.3; PO3.1; B7-2; Activation B7-2 antigen; B-lymphocyte activation antigen B7-2; B-lymphocyte antigen B7-2; B7.2; CD28 antigen ligand 2; CD28LG2; CD86; early T cell costimulatory molecule-1; early T-cell costimulatory molecule 1; ETC-1; LAB72; MGC34413; T-lymphocyte activation antigen CD86
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![Anti-CD86 [PO3]](/images/pub/media/catalog/product/NewWebsite/35/orb758797_1.png)
Immunofluorescence staining of fixed RAW264.7 cells with anti-CD86 antibody PO.3 (B467). Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of PO.3 (orb758797) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758797, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.
Quick Database Links
Gene Symbol
CD86
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
Anti-CD86 [PO3] (orb758797)
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