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Anti-CD98 [EP3-1]
Description
Images & Validation
−| Tested Applications | IF, IP |
|---|---|
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG |
| Clone No. | EP3-1 |
| Immunogen | Immunised mice and constructed IgG1 Fab phage display libraries from the spleen cells. CD98-reactive recombinant Fab fragments were retrieved by epitope peptide panning. |
| Target | CD98 |
| Purity | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
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![Anti-CD98 [EP3-1]](/images/pub/media/catalog/product/NewWebsite/35/orb1152499_1.png)
Flow cytometry using the Anti-CD98 antibody EP3-1. Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of EP3-1 (orb1152499, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
![Anti-CD98 [EP3-1]](/images/pub/media/catalog/product/NewWebsite/35/orb1152499_2.png)
Immunofluorescence staining of MCF7 cells with antiCD98 EP3-1. Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of EP3-1 (orb1152499) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1152499, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.
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Anti-CD98 [EP3-1] (orb1152499)
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