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Cdk6 Rabbit Polyclonal Antibody
SKU: orb371729
Description
Research Area
Cancer Biology, Cell Biology, Epigenetics & Chromatin, Protein Biochemistry
Images & Validation
−Item 1 of 3
| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human |
| Reactivity | Human, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Cdk6, identical to the related mouse sequence. |
| Target | Cyclin-dependent kinase 6 |
| Molecular Weight | 39 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 5mg rAlbumin, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Disclaimer | For research use only |
Alternative Names
−Cyclin-dependent kinase 6; 2.7.11.22; Cell division protein kinase 6; Serine/threonine-protein kinase PLSTIRE; CDK6; CDKN6
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IHC analysis of Cdk6 using anti-Cdk6 antibody. Cdk6 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Cdk6 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Cdk6 using anti-Cdk6 antibody. Cdk6 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Cdk6 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Cdk6 using anti-Cdk6 antibody. Cdk6 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Cdk6 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Quick Database Links
Gene Symbol
Cyclin-dependent kinase 6
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
Cdk6 Rabbit Polyclonal Antibody (orb371729)
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