Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	A synthetic peptide corresponding to a sequence in the middle region of human CNPase, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
Target	2',3'-cyclic-nucleotide 3'-phosphodiesterase
Molecular Weight	47 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 5mg rAlbumin, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Disclaimer	For research use only

Alternative Names

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2', 3'-cyclic-nucleotide 3'-phosphodiesterase; CNP; CNPase; 3.1.4.37; CNP

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Western blot analysis of CNPase using anti-CNPase antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. lane 1: rat brain tissue lysates, lane 2: mouse brain tissue lysates, lane 3: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CNPase antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CNPase at approximately 47KD. The expected band size for CNPase is at 47KD.

Quick Database Links

Gene Symbol

2',3'-cyclic-nucleotide 3'-phosphodiesterase

UniProt

P09543

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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