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Collagen 1 alpha 1 Rabbit Polyclonal Antibody
SKU: orb234810
Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | WB: 1:500-1000 |
| Reactivity | Bovine, Canine, Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Collagen 1 alpha 1. The exact sequence is proprietary. |
| Target | COL1A1 |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Collagen alpha-1(I) chain; Alpha-1 type I collagen
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Western blot analysis of Collagen 1 alpha 1 expression in EC9706 (A), A375 (B), SGC7901 (C), Hela (D), HEK293T (E) whole cell lysates. (Predicted band size: 138 kD; Observed band size: 130 kD)
Immunofluorescent analysis of Collagen 1 alpha 1 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
Collagen 1 alpha 1 Rabbit Polyclonal Antibody (orb234810)
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