Queen's Award Received in 2021 ISO 9001 Certified Delivered over 1,000,000 bio-reagents to life science researchers Trusted by Life Science Communities
Cart summary

You have no items in your shopping cart.

Cytokeratin 18 Antibody

SKU: orb43750

Description

Mouse monoclonal antibody to Cytokeratin 18

Research Area

Epigenetics, Infectious Diseases

Images & Validation

Tested ApplicationsELISA, FC, ICC, IHC-P, IP, WB
ReactivityHuman
Application Notes
Western blotting: Recommended dilution: 1-2 μg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: colon, skin. Immunocytochemistry: positive control: MCF-7 human breast adenocarcinoma cell line, HeLa human cervix carcinoma cell line. ELISA: Positive control: MCF-7 human breast adenocarcinoma cell line; the antibody DC-10 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04 (cat. no. orb43702). Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeMouse IgG1
Clone No.DC-10
ImmunogenHuman breast carcinoma cell line PMC-42.
TargetCytokeratin 18
PurificationPurified by protein-A affinity chromatography.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

K18, CK18, CYK18, KRT18

Similar Products

  • Cytokeratin 18/KRT18 Rabbit Polyclonal Antibody [orb389489]

    FC,  IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg
  • CK18 Mouse Monoclonal Antibody [orb499665]

    FC,  ICC,  IF,  IHC-Fr,  IHC-P,  WB

    Mouse, Rat

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μl, 200 μl, 200 μg, 50 μl
  • CK18 Recombinant Rabbit Monoclonal Antibody [orb500277]

    ICC,  IF,  IHC-Fr,  IHC-P,  WB

    Mouse, Rat

    Human, Mouse, Rat

    Rabbit

    Recombinant

    Unconjugated

    50 μl, 100 μl
  • CK18 Rabbit Polyclonal Antibody [orb5871]

    FC,  ICC,  IF,  IHC-Fr,  IHC-P,  KO/KD Validated,  WB

    Canine, Gallus, Rabbit

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl, 200 μl
  • Mouse Cytokeratin 18 / Keratin K18 Antibody [orb98259]

    FC,  ICC,  IHC-Fr,  WB

    Canine, Gallus, Hamster, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish

    Mouse

    Monoclonal

    Unconjugated

    0.1 mg
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
FC
Flow Cytometry
View Protocol
ICC
Immunocytochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
IP
Immunoprecipitation
View Protocol

Available Sizes

Select a size below

0.1 mg
$ 280.00
Free Secondary Antibody (20 ul)0/0

Please add an antibody product to your cart first.

DispatchUsually dispatched within 5-10 working days.
Bulk Enquiry