Anti-Cytokeratin 7 [OV-TL 12/30]

Flow cytometry using the anti-Cytokeratin 7 antibody OV-TL 12/30. HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG1 version of OV-TL 12/30 (orb758971, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed HeLa cells with anti-Cytokeratin 7 antibody OV-TL 12/30. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of OV-TL 12/30 (orb758971) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758971, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti- Cytokeratin 7 antibody OV-TL 12/30 HeLa(A) (0.00001 µg/ml), HepG2(B) (0.0003 ug/ml) and A549(C) (0.00001 µg/ml) cell lysate and human thyroid(D) (0.0003 ug/ml) and human placenta(E) (0.00001 µg/ml) tissue lysate (35 µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of OV-TL 12/30 (orb758971) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.