DDX5 Rabbit Polyclonal Antibody

SKU: orb97090

Description

Research Area

Epigenetics & Chromatin, Molecular Biology

Images & Validation

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Item 1 of 2

Tested Applications	FC, ICC, IF, IHC, IHC-Fr, WB
Dilution Range	Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human
Reactivity	Human, Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	A synthetic peptide corresponding to a sequence at the C-terminus of human DDX5, identical to the related rat and mouse sequences.
Target	Probable ATP-dependent RNA helicase DDX5
Molecular Weight	69 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Probable ATP-dependent RNA helicase DDX5; 3.6.4.13; DEAD box protein 5; RNA helicase p68; DDX5; G17P1, HELR, HLR1

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Western blot analysis of Lane 1: Rat Testis Tissue Lysate, Lane 2: Mouse Testis Tissue Lysate, Lane 3: HELA Cell Lysate, Lane 4: MCF-7 Cell Lysate, Lane 5: NIH3T3 Cell Lysate, Lane 6: SKOV Cell Lysate, Lane 7: MM231 Cell Lyate using DDX5 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Brain Tissue using DDX5 antibody

Quick Database Links

Gene Symbol

Probable ATP-dependent RNA helicase DDX5

UniProt

P17844

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-Fr

Immunohistochemistry Frozen

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Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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