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DR5 Antibody
SKU: orb1474321
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | WB (1/500 - 1/1000), IF/IC (1/50 - 1/100) |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | Purified recombinant human DR5 protein fragments expressed in E.coli. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−DR5; KILLER; TRAILR2; TRICK2; ZTNFR9; Tumor necrosis factor receptor superfamily member 10B; Death receptor 5; TNF-related apoptosis-inducing ligand receptor 2; TRAIL receptor 2; TRAIL-R2; CD262
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![Anti-DR5 [304]](/images/pub/media/catalog/product/NewWebsite/35/orb1463373_1.png)
![Anti-DR5 [304]](/images/pub/media/catalog/product/NewWebsite/35/orb1463373_2.png)
![Anti-DR5 [304]](/images/pub/media/catalog/product/NewWebsite/35/orb1463373_3.png)
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Western blot analysis of DR5 expression in mouse kidney (A), mouse brain (B), HCT116 (C), Hela (D) whole cell lysates. (Predicted band size: 48 kD; Observed band size: 48 kD)
Immunofluorescent analysis of DR5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
DR5 Antibody (orb1474321)
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