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EB2 Antibody
SKU: orb234827
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Description
Research Area
Epigenetics
Images & Validation
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| Tested Applications | IF, WB |
|---|---|
| Dilution range | WB: 1:500-1000 |
| Reactivity | Bovine, Gallus, Human, Monkey, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EB2. The exact sequence is proprietary. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Disclaimer | For research use only |
Alternative Names
−RP1; Microtubule-associated protein RP/EB family member 2; APC-binding protein EB2; End-binding protein 2; EB2
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Western blot analysis of EB2 expression in mouse heart (A), rat heart (B) whole cell lysates. (Predicted band size: 37 kD; Observed band size: 50 kD)
Immunofluorescent analysis of EB2 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
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Stefano Pezzini 1, Aurora Mustaccia 2, Pierre Aboa 1, Giorgia Faustini 1, Alessio Branchini 3, Mirko Pinotti 3, Angelisa Frasca 1, Joseph J Porter 4, John D Lueck 4, Nicoletta Landsberger Engineered tRNAs efficiently suppress CDKL5 premature termination codons Engineered tRNAs efficiently suppress CDKL5 premature termination codons, Sci Rep, (2024)
Applications
WB
Reactivity
Human
EB2 Antibody (orb234827)
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