Anti-EGFR [C225 (Cetuximab)]

SKU: orb1152521

Description

Mouse monoclonal antibody to EGFR

Research Area

Cancer Biology

Images & Validation

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Item 1 of 2

Tested Applications	Blocking, FC
Reactivity	Human

Key Properties

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Host	Human
Clonality	Monoclonal
Isotype	Human IgG1
Clone No.	C225 (Cetuximab)
Immunogen	The epidermal growth factor receptor is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands.
Target	EGFR
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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EGFR, ErbB-1, HER1; epidermal growth factor receptor

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Binding curve of anti-EGFR antibody to EGFR-Fc fusion protein ELISA Plate coated with human EGFR-Fc at a concentration of 2.0 mg/ml. A 2-fold serial dilution from 100 to 0.1 ng/ml was performed using HRP labelled cetuximab human IgG1 (orb348862).

Immunohistochemical staining of rat stomach tissue using anti-EGFR antibody C225 (Cetuximab) Anti-EGFR staining of formaldehyde fixed paraffin embedded rat stomach tissue, at 40x magnification. The human IgG1-chimeric version of C225 (orb348862) was used to stain samples at a concentration of 5 µg/ml.

Quick Database Links

Gene Symbol

EGFR

UniProt

P00533

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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