ERK1/2 Rabbit Monoclonal Antibody

SKU: orb1474616

Description

The ERK1/2 Antibody is suitable for IF, IP, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant protein of human ERK2 .Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

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Tested Applications	IF, IP, WB
Dilution Range	WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Monoclonal
Immunogen	Recombinant protein of human ERK2
Target	MAPK3; MAPK1
Purification	The antibody was purified by immunogen affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p44-ERK1; MAPK1; ERK2; PRKM1; PRKM2; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2

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Western blot analysis of ERK1/2 expression in K562 (A), rat brain (B), C6 (C), NIH3T3 (D), Hela (E) whole cell lysates. (Predicted band size: 44, 42 kD; Observed band size: 44, 42 kD)

Immunofluorescent analysis of ERK1/2 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

MAPK3; MAPK1

UniProt

P63085, P63086, P21708, Q63844, P27361, P28482

NCBI Gene ID

26417, 116590, 5594, 50689, 5595, 26413

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

Immunofluorescence

Immunoprecipitation