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Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)]
SKU: orb357996
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, FC, IF, IHC-P, WB |
|---|---|
| Reactivity | Virus |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG |
| Clone No. | D1-4G2-4-15 (4G2) |
| Immunogen | Dengue Virus type 2 antigens. |
| Target | Flavivirus group antigen |
| Purity | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Protein E; Envelope Protein
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Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)] [orb2645836]
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Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)] [orb357995]
ELISA, FC, IF, IHC-P, WB
Virus
Mouse
Monoclonal
Unconjugated
0.2 mg
![Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)]](/images/pub/media/catalog/product/NewWebsite/35/orb2645836_1.png)
![Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)]](/images/pub/media/catalog/product/NewWebsite/35/orb2645836_2.png)
![Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)]](/images/pub/media/catalog/product/NewWebsite/35/orb357995_1.png)
![Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)]](/images/pub/media/catalog/product/NewWebsite/35/orb357995_2.png)
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![Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)]](/images/pub/media/catalog/product/NewWebsite/35/orb357996_1.png)
Detection of Zika virus by immunofluorescence. Immunofluorescence images of Vero cells infected with ZIKV after 30h infection as well as controls. Cells were fixed in 4% PFA for 30 min at RT. Primary antibody (orb357996, rabbit IgG) staining was performed in 0.3% Triton X-100 at 1:20 dilution overnight at 4°C, detected using a Goat Anti-Rabbit IgG (AF568, 1:500) for 1h at RT and counterstained with DAPI.
![Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)]](/images/pub/media/catalog/product/NewWebsite/35/orb357996_2.png)
ELISA on Denge Virus-Like Particles using orb357995 Dengue Virus Serotype 2 VLPs were coated onto a plate at 5 µg/ml. Anti-flavivirus antibody (orb357995) added to plate in a 3-fold serial dilution starting at 1000 ng/ml. Detection performed using HRP labelled goat anti-mouse IgG.
Quick Database Links
Gene Symbol
Flavivirus group antigen
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)] (orb357996)
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