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Anti-GAPDH Mouse Monoclonal Antibody (2B5)
SKU: orb1147864
Description
Images & Validation
−Item 1 of 8
| Tested Applications | IF, IHC-P, WB |
|---|---|
| Reactivity | Canine, Gallus, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Yeast |
| Application Notes |
Key Properties
−| Clonality | Monoclonal |
|---|---|
| Isotype | Mouse IgG1 |
| Immunogen | Synthetic Peptide |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid solution |
| Buffer/Preservatives | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol. |
| Concentration | 1 mg/ml |
| Hazard Information | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Disclaimer | For research use only |
Alternative Names
−GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH
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HRP Conjugated Anti-GAPDH Mouse Monoclonal Antibody (2B5) [orb1147862]
IHC-P, WB
Canine, Gallus, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
Monoclonal
Unconjugated
200 μl, 50 μl, 5 x 250 μl
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Western blot analysis of Hela (1), rat brain (2), rabbit muscle(3), sheep muscle(4), and mouse brain (5), diluted at 1:10000.
Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.
Immunofluorescence analysis of human colon tissue. 1, GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Immunofluorescence analysis of mouse liver tissue. 1, GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Western blot analysis of Pig muscle tissue, GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25°C, 3h).
Western blot analysis of Hela, GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25°C, 3h).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
Anti-GAPDH Mouse Monoclonal Antibody (2B5) (orb1147864)
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