Anti-GITR [YGITR765]

SKU: orb613891

Description

Rat monoclonal antibody to GITR

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 2

Tested Applications	Blocking, FC
Reactivity	Mouse

Key Properties

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Host	Rat
Clonality	Monoclonal
Isotype	IgG2b
Clone No.	YGITR765
Immunogen	nB2/6TG cells transfected with mouse GITR injected into DA rats.
Target	GITR
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD357; Tumor necrosis factor receptor superfamily member 18; TNFRSF18; AITR; GITR-D; activation-inducible TNFR family receptor; AITR; glucocorticoid-induced tumor necrosis factor receptor

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Flow-cytometry using anti-GITR antibody YGITR765 Mouse activated T cells were stained with an isotype control (orb256390, Orange) or the rabbit-chimeric version of YGITR765 (orb613890) at a concentration of 1 µg/ml for 30 mins at RT without (Blue) and with (Grey) stimulation of the lymphocytes with immobilized anti-CD3 and anti-CD28 antibodies. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed mouse splenocytes with anti-GITR antibody YGITR765. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix™ coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of YGITR765 (orb613890) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613890, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.

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Gene Symbol

GITR

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Protocol Information

Flow Cytometry

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