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GPER Antibody
SKU: orb666812
Description
Research Area
Apoptotic, Cancer, Epigenetics, GPCR, Immunology, Neuroscience, Signaling Pathways
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | WB: 1-500-2000, IF: 1-50-200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Recombinant fusion protein of human GPER. The exact sequence is proprietary. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Disclaimer | For research use only |
Alternative Names
−CEPR; CMKRL2; DRY12; GPER; GPR30; G-protein coupled estrogen receptor 1; Chemoattractant receptor-like 2; Flow-induced endothelial G-protein coupled receptor 1; FEG-1; G protein-coupled estrogen receptor 1; G-protein coupled receptor 30; GPCR-Br; IL8-related receptor DRY12; Lymphocyte-derived G-protein coupled receptor; LYGPR; Membrane estrogen receptor; mER
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Western blot analysis of GPER expression in HeLa (A), rat lung (B) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 52 kD)
Immunofluorescent analysis of GPER staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
GPER Antibody (orb666812)
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