Anti-H4 K8Ac; K12Ac; K16Ac [KM-2]

SKU: orb758977

Description

Mouse monoclonal antibody to H4 K8Ac; K12Ac; K16Ac

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IF, IHC, WB
Reactivity	Human

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG2a
Clone No.	KM-2
Immunogen	KM-2 antibody was derived from an MRL/lpr lupus mouse.
Target	H4 K8Ac; K12Ac; K16Ac
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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histone 4; nucleosome; H4K8Ac; H4K12Ac; H4K16Ac; acetyl-Histone H4; Histone H4 acetylated at lysine; KM2

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Flow cytometry using the anti-H4 K8Ac; K12Ac; K16Ac antibody KM-2. HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG1 version of KM-2 (orb758978, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed HeLa cells with anti-H4 K8Ac; K12Ac; K16Ac antibody KM-2. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of KM-2 (orb758978) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom orb758978, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

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Gene Symbol

H4 K8Ac; K12Ac; K16Ac

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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