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Helios Antibody

SKU: orb222726

Description

Hamster monoclonal antibody to Helios

Research Area

Epigenetics

Images & Validation

Tested ApplicationsFC
ReactivityHuman, Mouse
Application Notes
Flow cytometry: Recommended dilution: 1-4 μg/ml.,Recommended protocol: 1) Perform staining of cell surface markers (CD25, CD4 etc.) for 20 min. at room temperature in the dark; 100 μl of peripheral blood. 2) Add 3 ml of PBS with 1% BSA, centrifugate at 300g and discard the supernatant. Further steps perform on ice and with ice-cold reagents. 3) Resuspend the cells in 5 ml of cold fixation solution (Miltenyi Biotec) and incubate for 30 min. on ice. 4) Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. 5) Resuspend the cells in 5 ml of ice-cold PBS with 1% BSA. 6) Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. 7) Resuspend the cells in 5 ml of ice-cold permeabilization solution (Miltenyi Biotec) and incubate 5 min. 8) Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. 9) Resuspend the cells in μl of ice-cold permeabilization solution and add 20 μl of FcR blocking solution and incubate for 5 min. (4°C, in the dark). 10) Perform intracellular staining of Helios for 30 min. (4°C, in the dark) with 10 μl of anti-Helios PE antibody (22F6) in 100 μl. 11) Add 2 ml of ice-cold permeabilization solution and incubate for 5 min. at 4°C in the dark. 12) Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. 13) Resuspend the cells in 3 ml of ice-cold PBS with 1% BSA. 14) Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. 15) Resuspend the cells in 150 μl of ice-cold PBS with 1% BSA and measure on a flow cytometry device with appropriate setting as soon as possible. Keep cold until measuring.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeArmenian Hamster IgG
Clone No.22F6
ImmunogenPeptide coresponding to the amino acids 51-107 of Helios
TargetHelios
PurificationPurified by protein-A affinity chromatography.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration1 mg/ml
DisclaimerFor research use only

Alternative Names

ANF1A2, IKZF2

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Helios Antibody

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-Helios (22F6) purified antibody (concentration in sample 8 μg/ml, GAM APC).

Helios Antibody

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-Helios (22F6) purified antibody (concentration in sample 8 μg/ml, GAM APC).

Helios Antibody

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-Helios (22F6) purified antibody (concentration in sample 8 μg/ml, GAM APC).

Helios Antibody

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-Helios (22F6) purified antibody (concentration in sample 8 μg/ml, GAM APC).

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

FC
Flow Cytometry
View Protocol

Helios Antibody (orb222726)

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0.1 mg
$ 340.00
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