Hepatitis B Virus Rabbit Polyclonal Antibody

SKU: orb18522

Description

Research Area

Cell Biology, Disease Biomarkers

Images & Validation

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Item 1 of 2

Tested Applications	IHC, WB
Dilution Range	Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Western blot, 0.1-0.5μg/ml, HBV
Reactivity	Human

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	A synthetic peptide corresponding to a sequence at the N-terminus of human Hepatitis B Virus.
Target	Large S protein
Molecular Weight	6 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 5mg rAlbumin, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Disclaimer	For research use only

Alternative Names

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Large S protein; S

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Hepatitis B Virus Rabbit Polyclonal Antibody

IHC analysis of Hepatitis B Virus using anti-Hepatitis B Virus antibody. Hepatitis B Virus was detected in paraffin-embedded section of human hepatitis B tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Hepatitis B Virus Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of Hepatitis B Virus using anti-Hepatitis B Virus antibody. Hepatitis B Virus was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Hepatitis B Virus Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Quick Database Links

Gene Symbol

Large S protein

UniProt

D2X4M3

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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