Histone H3 (Acetyl-K9) Rabbit Polyclonal Antibody

SKU: orb214780

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Description

The Histone H3 (Acetyl-K9) Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K9 of human Histone H3 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics & Chromatin

Images & Validation

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Item 1 of 5

Tested Applications	IF, IHC, WB
Dilution Range	WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
Reactivity	Bovine, Gallus, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K9 of human Histone H3 protein. The exact sequence is proprietary.
Target	HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; HIST2H3C; HIST2H3D; H3F3A; H3F3B
Molecular Weight	16 kDa
Purification	The antibody was purified by immunogen affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3/m; Histone H3/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3

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Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Western blot of mouse lung (1) using Histone H3 (Acetyl-K9) Rabbit Polyclonal Antibody orb214780 with dilution at 1:1000.

Immunohistochemical analysis of paraffin-embedded human lung cancer using Histone H3 (Acetyl-K9) Rabbit Polyclonal Antibody orb214780 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded rat kidney using Histone H3 (Acetyl-K9) Rabbit Polyclonal Antibody orb214780 with dilution at 1:100.

Immunofluorescent analysis of Histone H3 (Acetyl-K9) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; HIST2H3C; HIST2H3D; H3F3A; H3F3B

UniProt

P84245, P68431, P84244, Q6LED0, P68433, P84228, P84243, Q71DI3

NCBI Gene ID

291159, 126961, 8968, 15077, 100361558, 8358, 8350, 8351, 8353, 8354, 8355, 8352, 653604, 8356, 3020, 333932, 319152, 3021, 15078, 8357

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Western Blot (IB, immunoblot)

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IHC

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Immunofluorescence

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