Hsp27 HSPB1 Rabbit Monoclonal Antibody

SKU: orb547430

Description

Anti-Hsp27 HSPB1 Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Protein Biochemistry, Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications	FC, ICC, IF, IHC, IP, WB
Dilution Range	WB 1:500-2000 IHC 1:50-200 ICC/IF 1:50-200 IP 1:20 FC 1:20
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG
Clone No.	HII-8
Immunogen	A synthesized peptide derived from human Hsp27
Target	Heat shock protein beta-1
Molecular Weight	71 kDa
Purification	Affinity-chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Concentration	0.5mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; HSPB1; HSP27, HSP28

Phospho-HSP27 (Ser78) Recombinant Rabbit Monoclonal Antibody [orb559187]

Applications:	ELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Bovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat
Reactivity:	Human
Species/Host:	Rabbit
Clonality:	Recombinant
Conjugation:	Unconjugated

Sizes Available: 25 μl, 50 μl, 100 μl

Item 1 of 3
Anti-HSPB1 [SAIC-23C-35] [orb1089991]
Applications:
WB
Reactivity:
Human
Species/Host:
Rabbit
Clonality:
Monoclonal
Conjugation:
Unconjugated
Sizes Available: 0.2 mg
Item 1 of 3
Anti-HSPB1 [SAIC-23B-22] [orb1089992]
Reactivity:
Human
Species/Host:
Rabbit
Clonality:
Monoclonal
Conjugation:
Unconjugated
Sizes Available: 0.2 mg
Item 1 of 2
Anti-HSPB1 [SAIC-23E-30] [orb1089990]
Reactivity:
Human
Species/Host:
Rabbit
Clonality:
Monoclonal
Conjugation:
Unconjugated
Sizes Available: 0.2 mg

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

WB analysis of Hsp27 expression in HeLa Cell.

Quick Database Links

Gene Symbol

Heat shock protein beta-1

UniProt

P04792

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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Immunoprecipitation

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