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HSP60 Antibody
SKU: orb1474285
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IF, IP, WB |
|---|---|
| Dilution Range | WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50) |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | Purified recombinant human Hsp60 protein fragments expressed in E.coli. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−HSP60; 60 kDa heat shock protein mitochondrial; 60 kDa chaperonin; Chaperonin 60; CPN60; Heat shock protein 60; HSP-60; Hsp60; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein
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Western blot analysis of HSP60 expression in Hela (A), Raji (B), Jurkat (C), C6 (D), C2C12 (E), NIH3T3 (F) whole cell lysates. (Predicted band size: 61 kD; Observed band size: 61 kD)
Immunofluorescent analysis of HSP60 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
IP
Immunoprecipitation
HSP60 Antibody (orb1474285)
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