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Hsp70/HSPA1A/HSPA1B Rabbit Polyclonal Antibody
SKU: orb18121
Description
Research Area
Cancer Biology, Cell Biology, Protein Biochemistry, Signal Transduction
Images & Validation
−Item 1 of 5
| Tested Applications | FC, ICC, IF, IHC, IHC-Fr, WB |
|---|---|
| Dilution Range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat Immunocytochemistry , 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Hsp70, identical to the related rat and mouse sequence. |
| Target | Alpha-crystallin A chain |
| Molecular Weight | 70 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 5mg rAlbumin, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−HSP70-1; HSP70.1; HSPA1A; HSP72; HSPA1; HSX70; HEL-S-103; HSP70; HSP70-1A; HSP70-2; HSP70.2; HSP70I; HSP72; HSPA1; HSPA1B; HSP70-1B
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Western blot analysis of Lane 1: Rat Testis Tissue Lysate, Lane 2: Rat Brain Tissue Lysate, Lane 3: Rat Liver Tissue Lysate, Lane 4: Rat Lung Tissue Lysate, Lane 5: CEM Cell Lysate, Lane 6: HELA Cell Lysate, Lane 7: SMMC Cell Lysate, Lane 8: HT1080 Cell L
Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Brain Tissue using HSP70s antibody.
Immunohistochemical analysis of formalin-fixed paraffin embedded Human Mammary Cancer Tissue using HSP70s antibody.
Confocal immounofluoroscence analysis of HELA Cell using HSP70s antibody.
Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Intestine Tissue using HSP70s antibody.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
Hsp70/HSPA1A/HSPA1B Rabbit Polyclonal Antibody (orb18121)
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