HSP90 beta Antibody

SKU: orb1474297

Description

The HSP90 beta Antibody is suitable for IF, IP, WB. It is a Monoclonal, Unconjugated antibody which raised against Purified recombinant human Hsp90 beta protein fragments expressed in E.coli .Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

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Tested Applications	IF, IP, WB
Dilution Range	WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
Reactivity	Hamster, Human, Monkey, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant human Hsp90 beta protein fragments expressed in E.coli
Purification	The antibody was purified by immunogen affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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HSP90B; HSPC2; HSPCB; Heat shock protein HSP 90-beta; HSP 90; Heat shock 84 kDa; HSP 84; HSP84

Quality Guarantee

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Western blot analysis of HSP90 beta expression in Hela (A), NIH3T3 (B), C6 (C), CHOK1 (D), COS7 (E) whole cell lysates. (Predicted band size: 83 kD; Observed band size: 90 kD)

Immunofluorescent analysis of HSP90 beta staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Quick Database Links

UniProt

P34058, P08238, P11499

NCBI Gene ID

15516, 301252, 3326

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

Immunofluorescence

Immunoprecipitation