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CD105 Antibody
SKU: orb44185
Description
Research Area
Cancer, Cardiovascular Disease, Epigenetics, Stem Cells
Images & Validation
−Item 1 of 2
| Tested Applications | FC, ICC, IHC-Fr, WB |
|---|---|
| Reactivity | Human, Porcine |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a |
| Clone No. | MEM-229 |
| Immunogen | Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA. |
| Target | CD105 |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Endoglin, END, ENG, HHT1, ORW1
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Separation of HUVEC cells stained using anti-human CD105 (MEM-229) purified antibody (concentration in sample 3 µg/ml, red-filled, GAM APC) from HUVEC cells stained using mouse IgG1 isotype control (MOPC-21) purified antibody (concentration in sample 3 µg/ml, black-dashed, GAM APC) in flow cytometry analysis (surface staining).
Immunocytochemistry staining of an infarcted porcine heart with anti-CD105 (MEM-229; green); cell nuclei stained with DAPI (blue). Primary antibody dilution: 5 ug/ml.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
ICC
Immunocytochemistry
CD105 Antibody (orb44185)
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