CD138 Antibody (APC)

SKU: orb623578

Description

Mouse monoclonal antibody against Syndecan 1 conjugated to APC

Research Area

Epigenetics

Images & Validation

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Item 1 of 4

Tested Applications	FC
Reactivity	Human, Primate
Application Notes	Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG1 kappa
Clone No.	MI15
Immunogen	A mixture of U266 and XG-1 human myeloma cell lines
Target	CD138
Purification	Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Conjugation	APC

Storage & Handling

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Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/Preservatives	Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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syndecan 1, SYND1, SDC

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Staining pattern of Anti-human CD38 FITC antibody (clone HIT2) in dot-plot fluorescence visualization (B cell gate) Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. Mouse monoclonal anti-human CD38 FITC antibody (clone HIT2) in concentration 0.5 µg/ml in stained stained blood sample (2 x 10^6 cells) and Mouse monoclonal anti-human CD27 Pacific Blue™ antibody (clone LT27) was used in concentration 2 µg/ml, respectively.

Staining pattern of Anti-human CD38 FITC antibody (clone HIT2) in dot-plot fluorescence visualization (B cell gate) Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. Mouse monoclonal anti-human CD38 FITC antibody (clone HIT2) in concentration 0.5 µg/ml in stained stained blood sample (2 x 10^6 cells) and Mouse monoclonal anti-human CD138 APC antibody (clone MI15) in concentration 2 µg/ml, respectively.

Reactivity of Anti-human CD38 FITC antibody (clone HIT2) on human peripheral leukocytes Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. Suspension of blood leukocytes (2 x 10^6 cells) was added to the mixture of CD38 FITC antibody (clone HIT2, 0.5 µg/ml in stained blood sample), backbone antibody conjugates and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Stained sample was fixed with 2 ml of 10× diluted EXCELLYSE Easy solution for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Anti-human CD38 FITC antibody (clone HIT2) works in flow cytometry application. Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. Mouse monoclonal anti-human CD38 FITC antibody (clone HIT2) was used in concentration 0.5 µg/ml in stained blood sample (2 x 10^6 cells).

Quick Database Links

Gene Symbol

CD138

UniProt

P18827

NCBI Gene ID

6382

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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