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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 2
| Tested Applications | FC, ICC |
|---|---|
| Reactivity | Human, Primate |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 24-31 |
| Immunogen | human CD154 fusion protein |
| Target | CD154 |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD40L, CD40 ligand, TNFSF5, Ly62, gp39, T-BAM
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Separation of human CD154 positive cells (red-filled) from CD154 negative cells (black-dashed) in flow cytometry analysis (surface staining) of stimulated (PMA + ionomycin) peripheral blood mononuclear cells stained using anti-human CD154 (24-31) purified antibody (concentration in sample 2 μg/ml) GAM APC.
Flow cytometry surface staining pattern of human stimulated (PMA + ionomycin) peripheral blood mononuclear cells stained using anti-human CD154 (24-31) purified antibody (concentration in sample 2 μg/ml) GAM APC.
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Protocol Information
FC
Flow Cytometry
ICC
Immunocytochemistry
CD154 Antibody (orb305469)
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