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CD16 Antibody (PerCP)
SKU: orb154539
Description
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| Tested Applications | FC |
|---|---|
| Reactivity | Human, Primate |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 kappa |
| Clone No. | 3G8 |
| Immunogen | Human neutrophils |
| Target | CD16 |
| Purification | Purified antibody is conjugated with activated Peridinin-Chlorophyll Protein (PerCP) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Conjugation | PerCP |
Storage & Handling
−| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
|---|---|
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Disclaimer | For research use only |
Alternative Names
−FcgammaRIII, IGFR3, FCRIII
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Staining pattern of Anti-human CD16 PerCP antibody (clone 3G8) in dot-plot fluorescence visualization (lymphocyte gate). Analysis of the antibody staining profile was performed on blood leukocytes isolated from peripheral whole blood by bulk erythrocyte lysis using 10× diluted EXCELLYSE Live. Mouse monoclonal anti-human CD16 PerCP antibody (clone 3G8) was used in concentration 11 µg/ml in stained stained blood sample (2 x 10^6 cells) and Mouse monoclonal anti-human CD3 FITC antibody (clone UCHT1) in concentration 5 µg/ml, respectively.
Staining pattern of Anti-human CD16 PerCP antibody (clone 3G8) in dot-plot fluorescence visualization (mononuclear gate). Analysis of the antibody staining profile was performed on blood leukocytes isolated from peripheral whole blood by bulk erythrocyte lysis using 10× diluted EXCELLYSE Live. Mouse monoclonal anti-human CD16 PerCP antibody (clone 3G8) was used in concentration 11 µg/ml in stained stained blood sample (2 x 10^6 cells) and Mouse monoclonal anti-human CD14 APC-Cy™7 antibody (clone MEM-15) in concentration 4 µg/ml, respectively.
Reactivity of Anti-human CD16 PerCP antibody (clone 3G8) on human peripheral leukocytes. Analysis of the antibody staining profile was performed on blood leukocytes isolated from peripheral whole blood by bulk erythrocyte lysis using 10× diluted EXCELLYSE Live. Suspension of blood leukocytes (2 x 10^6 cells) was added to the mixture of CD16 PerCP antibody (clone 3G8, 11 µg/ml in stained blood sample), backbone antibody conjugates and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Stained sample was fixed with 2 ml of 10× diluted EXCELLYSE Easy solution for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.
Anti-human CD16 PerCP antibody (clone 3G8) works in flow cytometry application. Analysis of the antibody staining profile was performed on blood leukocytes isolated from peripheral whole blood by bulk erythrocyte lysis using 10× diluted EXCELLYSE Live. Mouse monoclonal anti-human CD16 PerCP antibody (clone 3G8) was used in concentration 11 µg/ml in stained blood sample (2 x 10^6 cells).
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CD16 Antibody (PerCP) (orb154539)
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