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CD1a Antibody (PE)
Description
Images & Validation
−| Tested Applications | FC |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | HI149 |
| Immunogen | Human thymocytes |
| Target | CD1a |
| Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Conjugation | PE |
Storage & Handling
−| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
|---|---|
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Disclaimer | For research use only |
Alternative Names
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Flow cytometry multicolor surface staining of human stimulated (GM-CSF + IL-4) peripheral blood monocytes stained using anti-human CD1a (HI149) PE antibody (20 µl reagent per milion cells in 100 µl of cell suspension) and anti-human CD11c (BU15) APC antibody (10 µl reagent per milion cells in 100 µl of cell suspension).

Separation of human CD1a positive CD11c positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from CD11c negative CD1a negative events (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood monocytes stained using CD1a (HI149) PE antibody (20 µl reagent per milion cells in 100 µl of cell suspension).

Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood monocytes stained using anti-human CD1a (HI149) PE antibody (20 µl reagent per milion cells in 100 µl of cell suspension).
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CD1a Antibody (PE) (orb43816)
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